KMID : 0939920200520030830
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´ëÇѾÏÇÐȸÁö 2020 Volume.52 No. 3 p.830 ~ p.847
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Activation of ¥â2-Adrenergic Receptor Promotes Growth and Angiogenesis in Breast Cancer by Down-regulating PPAR¥ã
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Zhou Jing
Liu Zhanzhao Zhang Lingjing Hu Xiao Wang Zhihua Ni Hong Wang Yue Qin Junfang
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Abstract
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Purpose: Chronic stress and related hormones are key in cancer progression. Peroxisome proliferator-activated receptor ¥ã (PPAR¥ã) and its agonists was reported that inducing anti-tumor effect. However, the function of PPAR¥ã in pro-tumorigenic effects induced by chronic stress in breast cancer remains unknown. Herein, we have characterized a novel role of PPAR¥ã and vascular endothelial growth factor (VEGF)/fibroblast growth factor 2 (FGF2) signals in breast cancer promoted by chronic stress.
Materials and Methods: We performed experiments in vivo and in vitro and used bioinformatics data to evaluate the therapeutic potential of PPAR¥ã in breast cancer promoted by stress.
Results: Chronic stress significantly inhibited the PPAR¥ã expression and promoted breast cancer in vivo. VEGF/FGF2-mediated angiogenesis increased in the chronic stress group compared to the control group. PPAR¥ã agonist pioglitazone (PioG) injection offset the pro-tumorigenic effect of chronic stress. Moreover, specific ¥â2-adrenergic receptor (¥â2R) antagonist ICI11-8551 inhibited the effect of chronic stress. In vitro, norepinephrine (NE) treatment had a similar tendency to chronic stress. The effect of NE was mediated by the ¥â2R/adenylate cyclase signaling pathway and suppressed by PioG. PPAR¥ã suppressed VEGF/FGF2 through reactive oxygen species inhibition. Bioinformatics data confirmed that therewas a lowPPAR¥ã expression in breast invasive carcinoma. Lower PPAR¥ã was associated with a significantly worse survival.
Conclusion: ¥â2R activation induced by chronic stress and related hormones promotes growth and VEGF/FGF2-mediated angiogenesis of breast cancer by down-regulating PPAR¥ã. Our findings hint that ¥â receptor and PPAR¥ã as two target molecules and the novel role for their agonists or antagonists as clinical medicine in breast cancer therapy
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KEYWORD
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¥â2 receptor, Chronic stress, PPAR¥ã, Norepinephrine, Breast neoplasms
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